抄録
AtGST11 is one of GST gene family that was induced by various stresses. Precise response mechanism of AtGST11 gene at transcriptional level during Al stress is now investigated in this study. A cDNA library was constructed from Arabidopsis treated with 0.1 mM Al and cloned in T7 phage dispay library. Phage lysate was hybridized with biotynelated AtGST11 promoter. This DNA/protein complex was captured using streptavidin paramagnetic particles. Fifty plaques were selected to determine the insert by PCR amplification followed by DNA sequencing. Ten plaques were determined to contain the desired inserts. Homology search against several databases revealed that one of these (#43) is a transcription factor HD-Zip encoded by ATHB6 gene which has known to be up-regulated to various abiotic stresses. This putative transcription factor is being confirmed by gel-shift assay. The existence of other proteins may as well suggest the transcription of AtGST11 involving complex factors acting as regulators.
