抄録
Peroxisomes are organelles present in most of eukaryotic cells and participate in the metabolism of fatty acids and other metabolites. Unlike chloroplasts or mitochondria, peroxisome proteins are synthesized in the cytosol and imported across their membranes. Thus there must be a peroxisome-specific protein transport system which is classified as peroxisome membrane protein transport and matrix protein import. Pex16 is a peroxisomal membrane protein that has been shown to regulate peroxisome biogenesis and membrane morphology. Pex7 is a soluble import receptor that recognizes peroxisomal targeting signal type 2 (PTS2)-containing proteins in the cytosol and transports them into peroxisomes across peroxisome membranes. Using transgenic plants overexpressing green fluorescence protein (GFP) tagged PEX7 and PEX16, we analyzed protein complexes of PEX7 or PEX16 proteins. By combination of co-immunoprecipitation technique and Orbitrap MS analysis, we identified 31 and 6 candidate proteins as binding partners of Pex7 and Pex16, respectively. Functional analysis of each candidate protein including the localization is under investigation for understanding peroxisomal biogenesis.
