抄録
In this study, formation of nitric oxide (NO) induced by Al, Cd, Cr, Cu and Zn was investigated in Al resistant Andropogon virginicus. The Al was semi-quantified with morin stain and found that mostly accumulates in the root tips, causing severe root growth retardation. Toxic symptoms such as chlorosis and growth retardation of shoot and roots were occurred by the tested metal stresses. Lipid peroxidation was detected in each stress and it was also found that as time increases lipid peroxidation increased. Furthermore, the production of NO due to the metal treatments was also observed by NO specific dye, DAF-FM. The observed results indicated that there was a difference in the NO production among the stresses. To confirm the NO production with these treatments, plants were treated with 0.3 mM of cPTIO. The NO scavenger made a significant reduction in the NO signal in the root tip region. We are now characterizing the role of produced NO by the metal stresses in the root tip region.
