抄録
We developed a handmade fluorometer which can measure the concentration of acetylsalicylic acid (ASA) in an aspirin tablet. ASA easily hydrolyzes to salicylic acid (SA) under the presence of hydroxide ions; SA is a fluorescent substance whose maximum excitation is 320nm. Therefore, black light (peak wavelength is 352nm) was adopted as the light source and a photodiode was used as the detector to measure the slight fluorescence of SA. The results show that the statistical dispersions by this new device were not larger and operations are easier than the titration method, which is widely accepted as the quantitative analysis of SA. Subsequently we held an experimental class by using both titration and fluorescent methods. In addition, we propose a teaching plan for introducing this fluorescence method into a science experiment in high school.
