土と微生物
Online ISSN : 2189-6518
Print ISSN : 0912-2184
ISSN-L : 0912-2184
岐阜県御嶽山の森林土壌から採取した菌核の培養法および 非培養法による菌類群集解析
野々山(佐々木) 弥坂上 伸生 成澤 才彦太田 寛行渡邊 眞紀子
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2016 年 70 巻 2 号 p. 56-59

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To investigate fungi involving sclerotium formation, we collected sclerotia from forest soils of Mt. Ontake, Gifu prefecture, Japan, and created the clone-library of their ITS regions of rDNA. We also cultured fungi from those sclerotia, followed by identifying fungal species. The results of the clone-library analyses suggested that Cenococcum geophilum, a fungus known for sclerotium formation, was included in the isolated fungi, which were mostly ascomycetes. Although culturing C. geophilum was not successful, three dark septate endophytes (DSE), all of which were unidentified, formed sclerotia on the artificial medium. Therefore, the sclerotia examined in this study might have been formed by not only C. geophilum but also by other fungi.

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