抄録
Thermophilic nitrifying Bacillus sp. T3 which had been isolated from cattle manure composts was capable of growing on a defined media containing high concentrations of sodium chloride. The addition of the thermophilic nitrifying bacteria to composts is expected for mitigation of ammonia volatilization loss during composting. Based on the results from assimilation tests of coloration, antibiotics and carbon sources, a selective medium was devised for the thermophilic nitrifying Bacillus sp. T3. The composition of the medium was as follows : ammonium sulfate, 5.0 g; sorbitol,1.0 g; yeast extract, 0.5 g; dipotassium hydrogen phosphate, 3.1 g; sodium chloride, 20.0 g; erythrosin, 50.0 mg; polymyxin B sulfate, 10.0 mg; streptomycin, 4.0 mg; agar, 15.0 g; deionized water, 1,000 ml; pH 8.0. The selection of the thermophilic nitrifying Bacillus sp. T3 on the devised selective medium is effective for its culture at 50 ℃. The effects of adding the thermophilic nitrifying Bacilluss sp. T3 in composting and soil solarization were evaluated by using the devised selective medium.
