論文
大気圧プラズマジェット照射によるリン脂質の分解
キーワード:
atmospheric-pressure plasma,
plasma medicine,
phospholipids,
lysophospholipids,
cell membrane
2015 年 36 巻 5 号 p. 257-262
詳細
2015 年 36 巻 5 号 p. 257-262
The aim of this study was to determine the effect of plasma irradiation, which is used in plasma medicine on biological tissues (cells). We used phospholipids, the main components of the cell membrane, namely, phosphatidylcholine (PC), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). Reference samples of the three phospholipids were irradiated with an atmospheric-pressure argon (Ar) plasma jet for 5s. The phospholipids irradiated with a plasma jet were separated by thin-layer chromatography (TLC). The TLC results indicated that several degradation products were obtained for PC and PG but not for PE after plasma jet irradiation. One of the degradation products obtained from PC was identified as lysophosphatidylcholine (LPC) and one of the products obtained from PG was identified as lysophosphatidylglycerol (LPG) by liquid chromatography-mass spectrometry. Therefore, PC and PG are degraded into their respective lysophospholipids by plasma jet irradiation, which disrupts the cell membrane.
