抄録
In the serological approach of typing various myxoviruses, fairly a variety of serum specimens, i.e. obtained at different stage of immunization from different animals, have been employed as the antibody without so serious precautions. However, in discussing the common antigenicity shared with various myxoviruses or in discussing the avidity of different antibodies to see the effect in experimental infections or in discussing the differea t susceptibility of antibody to the action of KIO4, as was the case at the outbreak of asian flu, it was thought to be an urgent problem to get information on the nature of antibodies obtained at different stage of immunization.
Thus the study was conducted using allantoic fluid culture of PR 8 virus as an antigen. The antigen was injected one to chicken intravenously, the other to guinea pig both intracardially and intranasally. Antibody response was tested for every 9 weeks. Only hemagglutination inhibition test was used for the antibody assay, but in the elaborated manner where the titer against wide range of hemagglutinin was examined to find out the serum of different avidity.
The main result obtained in this study will be summarized as follows:
1) In chicken, highest antibody titer was obtained at first week after intravenous injection. The antibody here obtained was of high avidity and resistant to the action of KIO4. Decrease in antibody titer as well as in avidity was found corresponding to the time elapse after the single antigen stimulus given intravenously.
2) In guinea pigs, even intracardial injection failed to produce antibody at early stage. 3 weeks were necessary to obtain the heighest titer, First week antibody obtained after intranasal injection was particular in its high sensitivity to the action of KTO4 and in its specific activity against the PR 8 virus, in the background where the other serum specimens so far tested reacted not only to the PR 8 strain but also to WS strain of influenza A virus. The first week antibody here obtained was also of low avidity.
Reinfection of guinea pig 8 weeks after the first infection resulted in the early antibody response. Moreover, antibody thus obtained was of high avidity.
