サルモネラにおけるO抗原15合成と34合成の関係

抄録

Phage ε³⁴ is a temperate phage, capable of converting group E₂ Salmonella (O antigens 3, 15) to group E₃ Salmonella (O antigens (3), (15), 34) by lysogenization. The formation of antigen 34 is accompanied by partial loss of antigens 3 and 15, suggesting a close relationship between antigens 34 and 3 or 15 (Nakagawa, 1957; 1959).
Since Salmonella anatum (group E₁, O antigens 3, 10) does not adsorb phage ε³⁴, its lysogenization with ε³⁴ was carried out under particular conditions suggested by the experiments by Uetake, Luria and Burrous (1958). The resulting ε³⁴-lysogenic cells (=A(ε³⁴)) are same as S. anatum in their O antigens and in their sensitivities to phages ε¹⁵ and ε³⁴, excepting their resistance to phage C₃₄₁. When they are infected with ε¹⁵, both antigens 15 and 34 appear within several minutes in infected cells.
Most remarkable are the findings that A (ε³⁴) cells do not form antigen 34, irrespective of the presence of prophage ε³⁴, which always leads to the formation of antigen 34 when it infects 3, 15 cells of group E₂, and that antigen 34 appears when A (ε³⁴) cells form antigen 15 by infection with phage ε¹⁵ These observations, together with Nakagawa's finding mentioned above, indicate that the formation of antigen 15, at least partially, is a necessary prerequisite for the formation of antigen 34. Antigen 3 does not seem to be necessary for the formation of antigen 34, since antigen 3 of S. anatum is identical with that of group E₂ cells or of A (ε¹⁵) cells but there is neither formation of antigen 34 nor loss of antigen 3 in A (ε³⁴).
The resistance of A (ε³⁴) to phage C₃₄₁ is considered to be analogous to the resistance of lysogenic strains to unrelated phages, which has been observed in phages P1, P2 and λ (Bertani, 1953; Benzer, 1955).