P-chloro-mercuribenzoate (PCMB) によるインフルエンザAウイルス (PR8 strain) の不活化
1960 年 10 巻 3 号 p. 154-156
詳細
1960 年 10 巻 3 号 p. 154-156
(1) By treating influenza A virus suspended in 0.1M phosphate buffer (pH 7) with 5×10-4M p-chloromercuribenzoate (PCMB) for 24 hours at 37°C, the infectivity which was measured by the membrane piece technique could be destroyed without affecting hemagglutinating capacity.
(2) When an equal volume of an aqueous solution (pH 7) of the reactivating agent, cysteine or sodium thioglycolate, within the initial 30 minutes of the inactivation period, was added to the PCMB-treated virus, the reactivation was obtained. When cysteine or sodium thioglycolate solution was added after 60 minutes of the inactivation period, essentially no reactivation was obtained.
From these results, it seemed that SH group of virus protein was necessary to manifest the infectivity, and it was concluded that the infectivity and the hemagglutinating capacity were the independent attributes respectively.
