BF1ウイルス: ウシから分離された新しい細胞病原性ウイルス

1. 分離と性状

抄録

Six viral agents were isolated in cultures of bovine embryonic renal cells from feces of apparently healthy cattle. These agents were concidered to represent strains of a single virus. The properties of the virus are as follows:
(1) The virus proliferates with cytopathogenic effect in cultures of kidneys, spleens, testicles, and ovaries of cattle, kidneys and lungs of bovine embryos, kidneys of pigs, rabbits, and guinea pigs, chicken embryo tissues, and HeLa cells. However, the virus demonstrates neither evidence of multiplication nor cytopathogenesis in cultures of horse, dog, and cat kidney cells.
(2) The virus is readily filtered through Berkefeld N and W.
(3) The virus is not completely inactivated by heating at 56°C for 120 minutes or at 60°C for 60 minutes, but its infectivity is completely lost at 65°C in 20 minutes.
(4) Storage at 37°C inactivated the virus in 42 days. The virus is very stable when stored at 4°, -20° or -80°C.
(5) Infectivity of the virus is maintained unchanged when the virus is frozen and thawed ten times.
(6) The virus is resistant to ethylether and trypsin.
(7) Infected materials are capable of agglutinating erythrocytes of sheep and horses at 4°C
(8) The virus has little clinical effect on suckling mice, but is serially maintained by intracerebral passages in this host.
(9) The virus has no pathogenecity to adult mice, and guinea pigs. Chicken embryos seem to support the virus multiplication but no pathological lesions are produced.
(10) Calves inoculated with the virus intracerebrally, intratracheally, intravenously, intraperitoneally, or perorally showed no clinical signs. However, further studies seem necessary regarding the pathogenecity of the virus in cattle.
(11) The six isolates are not distinguishable from each other by neutralization test. The virus shows no cross reaction in neutralization test with ECBO virus of Kunin and Minuse.
We have been unable to relate this virus to presently known viruses recovered from cattle and believe that it represents a hitherto undescribed virus. Hence, we designated it tentatively as the BF 1 virus. A wide dissemination of the virus among Japanese cattle was indicated by high incidence of neutralizing antibodies for BF 1 virus; 12% of 49 Holstein cattle in Hokkaido Pref., 71% of 31 cattle of Japanese breed in Shimane Pref., and 94% of 16 Jersey cattle inported from Australia had neutralizing antibodies.