抄録
It was the purpose of this paper to identify the complement fixing (CF) antigen from adenovirus type 3 particles, namely V antigen, and to compare the characteristics between V antigen and soluble antigen (S antigen). Some efforts have been made to describe the similarity and difference of these two antigens. Results were as follows:
1) In growth experiment of adenovirus type 3, infectivity titer in Hela cells began to rise in 21hours and the increase of CF titer was noted in 30hours, indicating that the infectivity titer preceded the CF titer. In extracellular media, however, both titers did not rise even after 48hours.
2) Virus particle and S antigen were separated through ultracentrifugation from adenovirus type 3 preparation. Virus particle repeatedly washed several times evidently showed reactivity to CF antibody. (“V antigen”.)
3) Experiements to compare “V antigen” and S antigen clarified that there was no difference in resistence to either heat or ether. Specificity of these two antigens was determined to be same by gel-diffusion method.
4) Destruction of virus particle by sonic oscillation resulted in the rise of CF titer. Virus particle treated by sonic oscillation formed a broader band in gel-diffusion method. These facts might indicate that S antigen was a part of virus particle constitution. But doubts still remained unsettled that S antigen was possibly adsorbed in the surface of virus particle or exsist as another particle sedimentable by ultra-centrifugation apart from virus particle itself.
5) Observation as long as 28 days was necessary for the determination of infectivity titer of adenovirus. Using this method for infectivity titration, it was not demonstrated that CF titer was high in comparison with infectivity titer in adenovirus.
