抄録
Horie, et al. reported that the animals intraperitoneally immunized with rabies virus had shown the protection to the intracerebral challenge of the virus, and was not always parallel to the neutralizing activity of the serum (Serum antibody), but was parallel to the neutralizing activity of the perfused brain-emulsion (Brain antibody).
In this paper an investigation of the characteristics of Brain antibody formed in mice and guinea pigs which intraperitoneally inoculated with the rabies virus (strain CVS) was presented.
The results are summarized as follows:
1) The approximate slope rates of neutralization lines of Brain antibody and Serum antibody were 2.0 and 1.0, respectively.
2) The slope of neutralization line of Brain antibody heated at 56°C for 30minutes was 2.0 and it was identical with the slope of the original non-heated Brain antibody.
3) The slopes of neutralization line of mouse Serum antibody added with the normal mouse brain-emulsion, and of neutralization line of guinea pig Serum antibody added with the guinea pig brain-emulsion immunized with Japanese B Encephalitis virus, and of neutralization line of the mouse brain-emulsion injected intracerebrally with the anti-rabies mouse serum, were identical with the slope of the original Serum antibody.
4) The slope of neutralization line of guinea pig Brain antibody added with the immune serum of guinea pigs immunized with Japanese B Encephalitis virus was identical with the slope of the original Brain antibody.
5) The Brain antibody blocking test was carried out by using the anti-globulin serum. As a result, it was shown that Brain antibody was blocked by the anti-globulin serum and its neutralizing capacity diminished.
6) The neutralizing capacity of Brain antibody was not influenced by the super centrifugation at 100, 000G for 1hour.
As stated above, both Brain antibody and Serum antibody had the characteristics as the antibody, but there was the certain difference between two neutralization lines. It was thought that the mechanism of neutralization reaction of both antibodies might not be analogous.
