非類縁ファージの溶原化に及ぼす影響

抄録

While in infection of cells of Salmonella anatum 293 (=A) with ε¹⁵[A], phage ε¹⁵ establishes lysogeny in about 3 hours after infection, in infection of cells A carrying prophage ε³⁴ cells which survive infection continue to segregate phage-carrier and noncarrier cells in their progeny even for several hours after infection. In contrast, however, when cells A(ε³⁴) are infected with ε¹⁵ which are propagated on A(ε³⁴), lysogeny is established in about 3 hours, even at a multiplicity of infection of 5 or less. These suggest a correlation between host-controlled variation and lysogenization.
When cells A are infected at first with ε¹⁵ and then 30 minutes later with ε³⁴, ε¹⁵ establishes lysogeny in about 3 hours without being affected by ε³⁴, indicating that the genetic function of ε³⁴ may not be responsible for the interference with prophage establishment of ε¹⁵[A] in cells A(ε³⁴). On the other hand, in this experiment, ε³⁴ is interfered with rapid establishment of lysogeny. And in contrast, ε³⁴ establishes lysogeny soon after infection when ε¹⁵-infected cells are infected with ε³⁴ 180 minutes after ε¹⁵-infection and/or when cells A(ε¹⁵) are infected with ε³⁴, indicating that the rapid prophage establishment of ε³⁴ is mainly attributed to the genetic properties of the phage itself.
There is no remarkable difference in time required to establish lysogeny between in infection of cells A and of cells A(g₃₄₁) with phage ε¹⁵[A(g₃₄₁)], indicating that prophage g₃₄₁ does not affect ε¹⁵ on its establishment of lysogeny.
Similarly, g₃₄₁ establishes lysogeny rapidly after infection of cells A as well as cells A(ε^y). It suggests that the rapid prophage establishment is attributed to the genetic properties of phage g₃₄₁.
The above findings lead to the conclusions that the process of lysogenization may be affected by the presence of the genome of unrelated phage.
Notes: A(ε³⁴), A(g₃₄₁), A(ε¹⁵), and A(ε^y) indicate strain A lysogenized with ε³⁴, g₃₄₁, ε¹⁵, and ε^y, respectively.
ε¹⁵[A], ε¹⁵[A(ε³⁴)], and ε¹⁵[A(g₃₄₁)] indicate phage ε¹⁵ propagated on strain A, A(ε³⁴), and A(g₃₄₁), respectively.