ギラン・バレー症候群を呈する多発性神経根炎患児より分離したCoxsackieウイルスに関する研究

第1篇 Coxsackieウイルスの分離並にその免疫血清学的検索

抄録

The attempt to type Coxsackie viruses isolated from the fecal specimens of cases with Guillain-Barré syndrome had disclosed relevant two strains Dohi (A-19) and Okumoto (A-2) in 1952. Further studies were made on 7 cases in 1953. Three strains Inaba (A-2), Kamada (A-2+A-5) and Saragai (B-1) were obtained in suckling mice. Pathogenicity of Inaba and Kamada strains revealed flaccid paralysis up to mice 10 days old with the finding of the generalized involvement of muscle. In contrast, Saragai strain exhibited spastic paralysis and tremor together with occasional cramp or jaundice up to mice 4 days old, and pathology revealed the involvement of fat tissue, pancreas, CNS, liver and muscle respectively.
Seven paired sera were concomitantly challenged with Inaba, Kamada, Kamada 2 (A-5), and Saragai strains in a measure of neutralization and complement fixation. Case 2 showed a rise in neutralizing antibody levels against A-2, and case 3 & 7 had the rise in B-1 titer. But case 1, 4 & 7 revealed a high titer against A-2 in the initial serum and a specimen taken in the convalescent stage showed the same titer. Case 5 & 6 being twin brothers of age 14 were with negative reading in titer. By and large, C-F antibodies appeared earlier in the acute sera and fall in the convalescent stadium. But in the cases with negative virus isolation, except for case 7 in Saragai strain, it seemed that the correlation of the results of C-F tests with those of the neutralization tests was obscure.
The significance of these agents in relation to the Guillain-Barré syndrome abundantly encountered in Okayama prefecture during 1952-53 would remain to await further investigation.