抄録
Evidence of double infection of two discernible types of group A Coxsackie virus has been obtained from a case with typical Guillain-Barré syndrome associated with eruptions. The onset of the girl of age 4 years was characterized by the flaccid paralysis of both legs. Four days later the measles-like eruptions appeared on upper arm which covered whole upper trunk within following 48 hours.
Isolation of viruses was scheduled from the fecal specimens of 7 days after onset by the method developed by Beeman et at. with the positive results. Typing of isolated this Kamada srtain with Coxsackie virus group A type 1-4 and Kudo (A-5) strains revealed the puzzling double character of A-2 and Kudo type. To determine whether this is the case of mixed infection or of undisclosed entity the separation of the viruses was accordingly performed by the method described by Huebner et al.
The mixing virus type at 5th passage level was reciprocally eliminated by the addition of corresponding single antiserum, whereas two pure types were independently identified as A-2 and A-5. Similar attemts from the original fecal specimens were repeatedly successful. Further cross-neutralization test was made with Inaba, Kamada-2 (5th passage suspension derived from 10-3 Kamada strain incubated with A-2 antiserum), Kudo and H-1 (A-5) strains.
The rise in antibody titer of paired Kamada sera was demonstrated in the reading from 1; 10 to 1; 100 against A-2 and equally 1; 100 against A-5.
The significance of the relevant double infection in this case to the symptome observed has not been obviated yet.
