レチノールとトコフェ口ール同族体の同時測定について

抄録

Simultaneous determination of retinol, α-tocopherol (α-toe), β-toeopherol ( β-toc), γ-toeopherol ( γ-toc), and δ-toeopherol (δ-toc) was performed by high-performanee liquid ehromatography ( HPLC). It had been difficult to separate β-toc and γ-toc completely when retinol and tocopherols were determined simultaneously by HPLC. A new reversed-phase column made it possible to separate β-toc and γ-toc completely with determination of retinol. The HPLC conditions were as follows, column: YMC-PACK A-302 S-5 120A ODS (Ø4.6mm×150mm),mobile phase: 70% isopropanol, sample solvent: 99.5% methanol, detector: UV (290nm), flow rate: 0.5ml/ min, temperature: ambient, and column pressure: 120Kg/cm². This method maybe useful to clarify the relationship between retinol and tocopherols.