The Journal of Toxicological Sciences
Online ISSN : 1880-3989
Print ISSN : 0388-1350
ISSN-L : 0388-1350
POTENTIALITY OF PROTAMINE SULFATE AS MUTAGEN
Golam SARWARDaiichi KAKIMOTOMizuo ONISHIRyoichi NAGATA
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Keywords: fish protamine
JOURNAL FREE ACCESS

1989 Volume 14 Issue 3 Pages 215-225

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Abstract

The mutagenicity of protamine sulfate has been clarified based on chromosomal aberration of cultured chinese hamster lung cells (cell line) in direct and metabolic activation methods and microbial mutagenesis (Ames test). In chromosomal aberration test, protamine sulfate caused cytotoxicity in the high doses (2500 and 5000 μg/ml) in the presence of rat liver homogenates (S-9). But very negligible or no cytotoxicity occurred in direct method at high dose (5000 μg/ml). Structural aberration of chromosome was not occurred in either of the methods. In microbial mutagenesis study, protamine sulfate did not show any cytotoxicity to microbes up to the dose of 5000 μg per plate. Furthermore, it did not have any effect in microbes like mutagens or like some toxic agent. The study reveals that protamine sulfate is not mutagen.

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