1989 Volume 11 Issue 4 Pages 461-479
Technical conditions were examined to obtain more intensified stainings of the enzyme activity band(s) of mosquito larvae after polyacrylamide gel electrophoresis by comparing buffer systems, pH conditions, metal ions, additives, and body parts. Improvements were achieved for several enzymes, making it easier to analyze biochemical polymorphism. For practical purposes, technical notes and revised recipes are given for the following 17 enzymes: ADH, LDH, MDH, ME, IDH, G6PD, Aox, XDH, AIP, AcP, Est, LAP, HK, Tre, Fum, PGM, and GPl.
