抄録
The purpose of this study was to clarify the effect of general anesthesia on blastogenesis of lymphocytes in dogs. Lymphocytes collected from the anesthetized dog were incubated with concanavalin A (Con A) or phytohemaggulutinin (PHA) mitogen. Blastogenesis of lymphocytes stimulated by these mitogens were evaluated by the stimulation index (S.I.) from the incorporation of3H-thymidine. In halothane anesthesia (GOF) group, each dog was induced anesthesia by mask inhalation of 4% halothane and maintained by 1% halothane through a tracheal tube for 2 hrs (Group A) . A significant decrease in S.I. value was indicated from 24 hrs to 4 weeks and the lowest value was recorded at 2 weeks after the anesthesia. In pentobarbital anesthesia group, receiving at a dose of 25 mg/kg intravenously (Group B), the lower values compared to those of pre-anesthesia were recognized at 24 hrs and 2 weeks after the anesthesia. The suppressive effect on blastogenesis of peripheral lymphocytes of halothane anesthesia was stronger than that of the pentobarbital anesthesia.
