Field Examination of Dried Live Vaccine of Kaeishi Virus Isolated from an Epizootic-Fever-Like Disease of Cattle

Abstract

A Kaeishi virus was isolated in the 1959 outbreak of an epizootic-fever-like disease among cattle in Japan. The virus was attenuated in chick embryo tissue culture cells for vaccine by Omori. The 33 rd passage virus of this line was made to dried live vaccine. The authors conducted field examination of this vaccine with cattle and obtained the following results.
1) The test dried live vaccine was applied with safety to milking cows, suckling calves, working oxen, and cows at the end of pregnancy. None of the vaccinated animals manifested any local reaction at the site of injection, fever, loss of appitite, diarrhea, abortion, and abnormal birth.
2) In all the vaccinated animals, neutralizing antibody increased in titer from 1: 4 to 1: 64 and was detectable from 115 to 379 days after vaccination.
3) It was considered that this vaccine was capable of producing neutralizing antibody enough to inhibit infection with this virus for one epizootic term.