Sulfadimethoxin is regarded in human medicine as a long-acting sulfonamide. It is characteristic of this drug that a high and prolonged blood level is maintained after medication of a low dosage of the drug. Veterinary medicine to administera high dosage of sulfonamide. In this examination, however, some effect was obtained from smaller dosages than those conventionally used for swine. The first method employed consisted of intramuscular injection of 20 mg per kg of body weight, which was followed by that of 10 mg/kg 8 hours later. In this case, blood levels of 3. 5-3. 9 mg% were maintained in swine 24 hours after the initial administration. In another method, swine were given a single dose of 40 mg/kg and showed blood levels of 2.7-3.5 mg% 24 hours after administration. In these trials, 10% solution of sulfadimethoxin in sterilized water was used. These methods gave good results in experimental treatment of bacterial diarrhea of swine in 91 cases. No marked side-effect was seen in any case.
A Kaeishi virus was isolated in the 1959 outbreak of an epizootic-fever-like disease among cattle in Japan. The virus was attenuated in chick embryo tissue culture cells for vaccine by Omori. The 33 rd passage virus of this line was made to dried live vaccine. The authors conducted field examination of this vaccine with cattle and obtained the following results. 1) The test dried live vaccine was applied with safety to milking cows, suckling calves, working oxen, and cows at the end of pregnancy. None of the vaccinated animals manifested any local reaction at the site of injection, fever, loss of appitite, diarrhea, abortion, and abnormal birth. 2) In all the vaccinated animals, neutralizing antibody increased in titer from 1: 4 to 1: 64 and was detectable from 115 to 379 days after vaccination. 3) It was considered that this vaccine was capable of producing neutralizing antibody enough to inhibit infection with this virus for one epizootic term.