Isolation of Peripheral Lymphocytes from New-born Calves by Density Gradient Centrifugation and Lymphocytic Response to Mitogen

Abstract

The densities of the separating fluid (Ficoll-Conray) of 1.073-1.077 g/ml were suitable for isolation of lymphocytes from the blood of neonatal calves. The mitogen-induced blastogenic response of lymphocytes isolated from 7 newborn calves was satisfactory when mea suredby DNA fluorometric assay.