High-Performance Liquid Chromatography Analysis of Serum from a Bovine Case of Jaundice with Splenomegaly

Abstract

A female five-year-old Holstein cow showed remarkable splenomegaly, petechiae in the kidney and yellowcolored carcasses at meat inspection, and microbiologicaly diagnosed as septicemia. Serum was subjected to high-performance liquid chromatography using an internal-surface reversed-phase silica support column revealed jaundice with predominance of unconjugated bilirubin.