Abstract
On January 9, 2004, 38 Holstein cows bred in Fukuoka Prefecture showed signs of respiratory symptoms with diarrhea. Milk production had diminished rapidly, and Bovine coronavirus (BCV) was initially isolated from nasal cavity swabs. After a two-week period of testing, the resulting rise in hemagglutination inhibition antibody titer (HI-titer) positively identified the presence of BCV. When testing for Bovine RS virus and Infectious Bronchitis Respiratory virus, neither could be isolated or confirmed by their antigen. Their resulting antibody titer did not rise, and no likely bacteria or parasites matching their symptoms were present in this case. BCV was therefore identified as the primary cause of the dairy cattle's respiratory disease.
