Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Isolation of Enteric Bacteria, and Immunohistochemical Detection of the Bacterial Antigens from Milk-spotted Livers of Pigs
Satoshi MURAKAMIRyozo AZUMAEiji YOKOYAMAHiroaki OOMITakemi OBAShinichi SUZUKIKouhei TAKASAKI
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2007 Volume 60 Issue 4 Pages 295-298

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Abstract
Five milk-spotted livers (field cases), three normal livers from slaughter house, five samples from Ascaris suum-infected pigs (experimental cases) and three control cases were examined for bacterial isolation and immunohistochemical detection of enteric bacteria. Several kinds of bacteria were isolated from all cases. The total number of isolated bacteria from field cases was larger than that from normal livers, and the difference was statistically significant. Furthermore, Escherichia coli were isolated from all field cases and one experimental case. The immunolabelling gram-negative rod bacteria were found in hepatic sinusoids, the interlobular bile ducts and Glisson's sheath in 2 field cases and one experimental case. It i suggested that large number of isolated bacteria from field cases is associated with larval migrations in liver, although we could not find the relationship between the immunohistochemical distribution of positive antigens of enteric bacteria and hepatic white spot lesions.
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