Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Detection of Clostridium septicum Hemolysin Gene by Polymerase Chain Reaction
Shotaro TAKEUCHINaoko HASHIZUMETakafumi KINOSHITAToshio KAIDOHYutaka TAMURA
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1997 Volume 59 Issue 9 Pages 853-855

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Abstract

A polymerase chain reaction (PCR) was developed for the detection of the hemolysin (alpha toxin) gene of Clostridium septicum. The PCR primers were designed from the sequence of the hemolysin gene and synthesized. A DNA fragment of 270 bp was amplified from10 strains of C. septicum, but was not from strains of C. chauvoei, C. perfringens, C. novyi, or C. haemolyticum. When the PCR product was digested with Sau3AI, two DNA fragments of the expected 148 bp and 122 bp were recognized. The lowest detectable threshold of PCR for the hemolysin gene was 3.8 × 10 3 cells/ml. The PCR technique may be useful for rapid detection or identification of C. septicum associated with malignant edema.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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