New Quantitative Methods for Detection of Feline Parvovirus (FPV) and Virus Neutralizing Antibody against FPV Using a Feline T Lymphoid Cell Line

Yasuhiro IKEDA; Takayuki MIYAZAWA; Kyoko KUROSAWA; Risako NAITO; Shinichi HATAMA; Chieko KAI; Takeshi MIKAMI

doi:10.1292/jvms.60.973

Yasuhiro IKEDA, Takayuki MIYAZAWA, Kyoko KUROSAWA, Risako NAITO, Shinichi HATAMA, Chieko KAI, Takeshi MIKAMI

Author information

Yasuhiro IKEDA
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Takayuki MIYAZAWA
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Kyoko KUROSAWA
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Risako NAITO
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Shinichi HATAMA
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Chieko KAI
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Takeshi MIKAMI
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Keywords: CPV, FPLV, neutralization test

JOURNAL FREE ACCESS

1998 Volume 60 Issue 8 Pages 973-974

DOI https://doi.org/10.1292/jvms.60.973

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Abstract

Previously, we reported that a feline T lymphoid cell line, FL74 cells, was very sensitive to feline parvovirus (FPV) infection. In the present study, we developed new quantitative methods for detection of FPV and virus neutralizing antibody against FPV using FL74 cells. The methods presented here were very simple and applicable to both canine parvovirus and feline panleukopenia virus.

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