Effects of Isoprothiolane on Cell Growth of Cultured Bovine Mammary Epithelial Cells

Hiroyuki Okada; Yuriko Miyake; Hiromichi Ohtsuka; Yoshio Kiku; Shigeo Fukuda; Atsushi Watabane; Yuichi Yokomizo; Thomas J. Rosol; Tomo-o Yoshino

doi:10.1292/jvms.61.553

Hiroyuki Okada, Yuriko Miyake, Hiromichi Ohtsuka, Yoshio Kiku, Shigeo Fukuda, Atsushi Watabane, Yuichi Yokomizo, Thomas J. Rosol, Tomo-o Yoshino

Author information

Hiroyuki Okada
Department of Veterinary, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan
Yuriko Miyake
Department of Veterinary, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan
Hiromichi Ohtsuka
Department of Veterinary Teaching Hospital, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan
Yoshio Kiku
Department of Veterinary, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan
Shigeo Fukuda
Department of Veterinary, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan
Atsushi Watabane
Hokkaido Branch, National Institute of Animal Health, Sapporo, Hokkaido 062-0045, Japan
Yuichi Yokomizo
National Institute of Animal Health, 3-1-1 Kan-nondai, Tsukuba, Ibaraki 305-0856, Japan
Thomas J. Rosol
Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210-1093, U.S.A.
Tomo-o Yoshino
Department of Veterinary, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan

Keywords: cytokine, isoprothiolane, mammary gland

JOURNAL FREE ACCESS

1999 Volume 61 Issue 5 Pages 553-556

DOI https://doi.org/10.1292/jvms.61.553

Details

Abstract

This study was performed to investigate the effects of isoprothiolane on cell growth and the production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. Isoprothiolane increased proliferation of mammary epithelial cells in a dose-dependent manner at the concentration of 0.05 to 5 μM when cultured either with or without serum-supplemented medium. In contrast, isoprothiolane (0.0005-5 μM) significantly inhibited the production of IL-1 and IL-6 by mammary epithelial cells. Moreover, the cytokines, IL-1α, IL-1β, IL-6, and tumor necrosis factor (TNF)-α tended to inhibit the proliferation of mammary epithelial cells in a dose-dependent manner. These results indicated that isoprothiolane regulated mammary epithelial cell growth in vitro possibly by modulating the production of cytokines.

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