Abstract
Monoclonal antibodies(Mabs)against bovine α1−acid glycoprotein(α1AGP)were prepared from mouse hybridoma cell line.Bovine α1AGP as antigen was purified by using ion exchange column chromatography and the yield from 500 ml serum was about 10 mg.Immunoglobulin isotypes of 3 Mabs obtained were IgM and light chain types were κ.The Mabs reacted with bovine α1AGP on immunoblot analysis, but not with α1AGP digested with N−glycosidase, suggesting that an epitope recognized by these Mabs may be associated with a glycan side chain of bovine α1AGP.
