Antibody Reactivity to Cryptosporidium parvum in Saliva of Calves after Experimental Infection

Atsuko TOYOGUCHI; Yoshitaka OMATA; Tomohiro KOYAMA; Takeshi KAMIYOSHI; Keisei TAKEDA; Hidefumi FURUOKA; Yoshiyasu KOBAYASHI; Fudeko KAKUDA; Ryuichiro MAEDA; Takane MATSUI; Atsushi SAITO; Takeshi MIKAMI

doi:10.1292/jvms.62.1231

Atsuko TOYOGUCHI, Yoshitaka OMATA, Tomohiro KOYAMA, Takeshi KAMIYOSHI, Keisei TAKEDA, Hidefumi FURUOKA, Yoshiyasu KOBAYASHI, Fudeko KAKUDA, Ryuichiro MAEDA, Takane MATSUI, Atsushi SAITO, Takeshi MIKAMI

Author information

Atsuko TOYOGUCHI
Departments of Veterinary Physiology,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Yoshitaka OMATA
Departments of Veterinary Physiology,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Tomohiro KOYAMA
Departments of Veterinary Physiology,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Takeshi KAMIYOSHI
Departments of Veterinary Physiology,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Keisei TAKEDA
Departments of Veterinary Physiology,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Hidefumi FURUOKA
Departments of Veterinary Physiology,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Yoshiyasu KOBAYASHI
Departments of Veterinary Pathology,Obihiro Univiersity of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Fudeko KAKUDA
ORGANO Ltd.,4-9,Kawagishi,Toda,Saitama 335-0015,Japan
Ryuichiro MAEDA
Departments of Veterinary Physiology,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Takane MATSUI
Departments of Veterinary Pathology,Obihiro Univiersity of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Atsushi SAITO
Departments of Veterinary Physiology,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan
Takeshi MIKAMI
The National Research Center for Protozoan Diseases,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro 080-8555,Japan

Keywords: Cryptosporidium parvum, IgA, saliva

JOURNAL FREE ACCESS

2000 Volume 62 Issue 11 Pages 1231-1234

DOI https://doi.org/10.1292/jvms.62.1231

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Abstract

Antibodies against Cryptosporidium parvum in the saliva and sera of three calves experimentally infected with this parasite were examined by an indirect immunofluorescence antibody test and immunoblotting.Salivary anti−C.parvum IgA antibody appeared on day 12 post−challenge and had a tendency to increase transiently between days 15 and 30 post−challenge.Salivary anti−C.parvum IgG antibody levels showed a gradual increase along with the change in IgA antibody levels during the infection.In contrast, serum anti−C.parvum IgA antibody levels showed only a slight increase between days 15 and 30 post−challenge.Serum anti−C.parvum IgG antibody levels rose on day 12 post−challenge and one calf maintained relatively high level up to the end of the experiment.In immunoblotting, an antigen with a molecular mass of 15 kDa was found to react strongly to salivary IgA antibody and a 27 kDa antigen to react to serum IgG antibody.

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