Retinoid Receptors and the Induction of Apoptosis in Canine Osteosarcoma Cells

Sung-Hyeok HONG; Emi OHASHI; Tsuyoshi KADOSAWA; Manabu MOCHIZUKI; Satoru MATSUNAGA; Ryohei NISHIMURA; Nobuo SASAKI

doi:10.1292/jvms.62.469

Sung-Hyeok HONG, Emi OHASHI, Tsuyoshi KADOSAWA, Manabu MOCHIZUKI, Satoru MATSUNAGA, Ryohei NISHIMURA, Nobuo SASAKI

Author information

Sung-Hyeok HONG
Laboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Emi OHASHI
Laboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Tsuyoshi KADOSAWA
Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Manabu MOCHIZUKI
Laboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Satoru MATSUNAGA
Veterinary Medical Center, Faculty of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8659, Japan
Ryohei NISHIMURA
Laboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Nobuo SASAKI
Laboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

Keywords: osteosarcoma, receptor, retinoids

JOURNAL FREE ACCESS

2000 Volume 62 Issue 4 Pages 469-472

DOI https://doi.org/10.1292/jvms.62.469

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Abstract

Retinoids, all-trans-retinoic acid (ATRA) and 9-cis-retinoic acid (9-cis-RA), induced morphological changes and apoptosis-like cell death characterized by cell shrinkage, chromatin condensation and nuclear disintegration in three canine osteosarcoma cells, OOS, HOS and POS, at a concentration of 10^-5 M. Both retinoid receptors, RARs and RXRs, were identified in these cells. 9-cis-RA bound to both the RXRs and the RARs, whereas ATRA bound to only the RARs in these cells. Those results indicate that the induction of apoptosis in canine osteosarcoma cells may be mediated by the specific control of RARs and RXRs.

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