Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
Cloning and Chromosomal Assignment of the Porcine Interleukin-2 Receptor Alpha (IL-2Rα) Gene
Takehiro KOKUHOHideki HIRAIWAHiroshi YASUESatoko WATANABEYuichi YOKOMIZOShigeki INUMARU
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JOURNALS FREE ACCESS

2000 Volume 62 Issue 8 Pages 841-847

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Abstract

Porcine genomic DNA encoding a 55 kDa subunit of interleukin-2 receptor (IL-2R), which is termed α chain (IL-2R α), was cloned by repeated plaque hybridization using IL-2Rα cDNA as a probe. Two different lambda phage clones, one of which encoded exon 1 and the 5'-upstream flanking region of IL-2Rα gene and another encoded the sequence from exon 2 to exon 8, were isolated. By analysis of the 5'-upstream region of the gene, putative binding motifs for transcription factors such as GATA family proteins, Ikaros, NF-κB, NF-IL2R α and SRF, were found as described in human, murine and bovine genes. Two additional motifs for STAT4 binding were also found in this region. Moreover, using the FISH technique, we assigned the porcine IL-2Rα locus to the distal end of the long arm of chromosome 10 (10q6-qter) where the vimentin gene had been assigned nearby.

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