Pathogenicity and Gene Analysis of Adenovirus from Pigeons with Inclusion Body Hepatitis

Abstract

The pathogenicity of an adenovirus isolated from pigeons(Pigeon adenovirus, PA)with inclusion body hepatitis in Taiwan was investigated in specific−pathogen−free(SPF)chicks and in racing pigeons.One−day−old SPF chicks were inoculated subcutaneously with 10⁴ 50% tissue culture infective dose(TCID₅₀, low dose)and 10⁸ TCID₅₀(high dose)of the virus, respectively.The chicks began to die three days post inoculation(DPI)with high dose of the virus and the mortality reached 100%;the chicks began to die 6 DPI and the mortality reached 90% at 14 DPI with low dose.The adult pigeons seemed to be resistant to the PA.However, this virus decreased the production of antibody against Newcastle disease virus in pigeons.It is found that this PA belongs to genetic group D from the restriction patterns produced by BamH I and Hind III.