Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Detection of Antibodies against Pasteurella multocida Using Immunohistochemical Staining in an Outbreak of Rabbit Pasteurellosis
Hisayuki TAKASHIMAHiroki SAKAITokuma YANAIToshiaki MASEGI
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2001 Volume 63 Issue 2 Pages 171-174

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Abstract

To detect serum antibody against Pasteurella multocida (P.multocida) in infected rabbits, a modified immunoperoxidase assay was applied. An outbreak of P.multocida infection in rabbits started from sudden death. The infected rabbits had severe fibrinous and purulent pneumonia with hemorrhage, and a large number of P.multocida (A:12) was isolated from the trachea and lungs of the animals. Antibodies of IgM and IgG to P.multocida were assessed by immunohistochemical staining using the sera of the animals as primary antibodies and applying them to formalin-fixed, paraffin-embedded sections of P.multocida attached to calf fibrin. IgM antibodies to P.multocida were first detected 7 days after the onset of the disease. IgG antibodies began to rise on the 7th or 14th day. These results suggested that the modified immunoperoxidase assay could detect antibodies against P.multocida.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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