Cytotoxic T-Lymphocyte Activity Specific for Hemagglutinin (H) Protein of Canine Distemper Virus in Dogs

Kyoko HIRAMA; Ken-ichi TOGASHI; Chiaki WAKASA; Misako YONEDA; Toshiya NISHI; Yasuyuki ENDO; Ryuichi MIURA; Kyoko TSUKIYAMA-KOHARA; Chieko KAI

doi:10.1292/jvms.65.109

Kyoko HIRAMA, Ken-ichi TOGASHI, Chiaki WAKASA, Misako YONEDA, Toshiya NISHI, Yasuyuki ENDO, Ryuichi MIURA, Kyoko TSUKIYAMA-KOHARA, Chieko KAI

著者情報

Kyoko HIRAMA
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo
Ken-ichi TOGASHI
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo
Chiaki WAKASA
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo
Misako YONEDA
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo
Toshiya NISHI
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo
Yasuyuki ENDO
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo
Ryuichi MIURA
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo
Kyoko TSUKIYAMA-KOHARA
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo
Chieko KAI
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo

キーワード: canine distemper virus, cytotoxic T-lymphocyte, hemagglutinin

ジャーナルフリー

2003 年 65 巻 1 号 p. 109-112

DOI https://doi.org/10.1292/jvms.65.109

詳細

抄録

Cytotoxic T-lymphocyte (CTL) responses to hemagglutinin (H) protein of canine distemper virus (CDV) were evaluated in dogs using the replication-deficient adenovirus protein expression system. Skin fibroblasts were isolated from two dogs and were infected with recombinant adenovirus bearing the CDV-H gene (Ade-CDVH). CTL assay was performed using fibroblasts expressing CDV-H protein as target cells and peripheral blood lymphocytes (PBL) collected from the same dogs one week after immunization of CDV as effector cells. Specific cytotoxic activity was observed against autologous but not heterologous fibroblasts expressing CDV-H protein. These results indicate that the CTL epitope(s) were localized in the H protein.

責任著者(Corresponding author)

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