Abstract
Multiple necrotic hepatitis lesions of 5 newly hatched broiler chicks in three flocks derived from two hatcheries were examined pathologically. The livers were brittle, and multiple yellowish or green foci were scattered on the surface and cut surface. The main histological finding was well demarcated multi-focal necrosis in the liver. Many Gram-positive large bacilli that reacted positively with polyclonal anti-Clostridium perfringens serum were observed in necrotic areas.