Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
NOTE
Influence of Theophylline Supplementation on Oocyte Penetration of Canine Epididymal Spermatozoa
Mitsugu HISHINUMAJunjiro SEKINE
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2004 Volume 66 Issue 11 Pages 1417-1419

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Abstract

The influence of supplementation of theophylline to the medium regarding the penetration of canine epididymal spermatozoa into immature oocytes was examined. In the control medium, sperm penetration into the oocytes was observed in 8 of 13 dogs (61.5%), and the mean penetration rate was 22.0%. The penetration rate of individual dogs ranged from 0 to 64.9%. Supplementation of 0.1, 1.0 and 2.5 mM theophylline to the medium did not significantly affect sperm penetration. Sperm penetration was induced by supplementation of 2.5 mM theophylline in two dogs that showed no sperm penetration in the control group. Penetration of the epididymal spermatozoa into the oocytes was shown to vary among individual dogs in cases of the absence and presence of theophylline.

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© 2004 by the Japanese Society of Veterinary Science

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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