Improvement of Anemia Associated with Chronic Renal Failure by Recombinant Human Erythropoietin Treatment in ICR-Derived Glomerulonephritis (ICGN) Mice

Misuzu YAMAGUCHI-YAMADA; Noboru MANABE; Yasufumi GOTO; Sayuri ANAN; Keiko MIYAMOTO; Youhei MIYAMOTO; Masaya NAGAO; Yoshie YAMAMOTO; Atsuo OGURA

doi:10.1292/jvms.66.883

NOTE

Improvement of Anemia Associated with Chronic Renal Failure by Recombinant Human Erythropoietin Treatment in ICR-Derived Glomerulonephritis (ICGN) Mice

Misuzu YAMAGUCHI-YAMADA, Noboru MANABE, Yasufumi GOTO, Sayuri ANAN, Keiko MIYAMOTO, Youhei MIYAMOTO, Masaya NAGAO, Yoshie YAMAMOTO, Atsuo OGURA

Author information

Misuzu YAMAGUCHI-YAMADA
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
Noboru MANABE
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
Yasufumi GOTO
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
Sayuri ANAN
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
Keiko MIYAMOTO
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
Youhei MIYAMOTO
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
Masaya NAGAO
Laboratory of Biosignals and Response, Department of Applied Molecular Biology, Kyoto University
Yoshie YAMAMOTO
Department of Veterinary Sciences, National Institute of Infectious Diseases
Atsuo OGURA
Bioresource Center, RIKEN

Keywords: anemia associated with chronic renal failure, erythropoietin, hereditary nephrotic mouse (ICGN)

JOURNAL FREE ACCESS

2004 Volume 66 Issue 7 Pages 883-886

DOI https://doi.org/10.1292/jvms.66.883

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Abstract

The ICR-derived glomerulonephritis (ICGN) mouse, a novel inbred mouse strain with a hereditary nephrotic syndrome, develops severe anemia associated with chronic renal failure. To reveal the pathogenic mechanism of anemia in ICGN mice, we subcutaneously administered recombinant human erythropoietin (rhEPO; 5 IU/mouse/day) or saline for 5 days to ICGN mice. In terminal-stage ICGN mice with severe anemia, rhEPO significantly increased hematocrit (Ht), red blood cells (RBC) and hemoglobin levels. Endogenous EPO levels in peripheral blood were reduced by rhEPO injection. No histopathological changes in bone marrow and kidneys were induced by rhEPO injection. Insufficiency of EPO may cause anemia in ICGN mice.

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