Antibabesial Activity of Protoberberine Alkaloids and 20-Hydroxyecdysone from Arcangelisia flava against Babesia gibsoni in Culture

SUBEKI; Hideyuki MATSUURA; Kosaku TAKAHASHI; Masahiro YAMASAKI; Osamu YAMATO; Yoshimitsu MAEDE; Ken KATAKURA; Mamoru SUZUKI; TRIMURNINGSIH; CHAIRUL; Teruhiko YOSHIHARA

doi:10.1292/jvms.67.223

NOTE

Antibabesial Activity of Protoberberine Alkaloids and 20-Hydroxyecdysone from Arcangelisia flava against Babesia gibsoni in Culture

SUBEKI, Hideyuki MATSUURA, Kosaku TAKAHASHI, Masahiro YAMASAKI, Osamu YAMATO, Yoshimitsu MAEDE, Ken KATAKURA, Mamoru SUZUKI, TRIMURNINGSIH, CHAIRUL, Teruhiko YOSHIHARA

Author information

SUBEKI
Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Hideyuki MATSUURA
Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Kosaku TAKAHASHI
Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Masahiro YAMASAKI
Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Osamu YAMATO
Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Yoshimitsu MAEDE
Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Ken KATAKURA
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
Mamoru SUZUKI
Department of Parasitology, Gunma University School of Medicine
TRIMURNINGSIH
Research and Development for Biology, Indonesian Institute of Sciences
CHAIRUL
Research and Development for Biology, Indonesian Institute of Sciences
Teruhiko YOSHIHARA
Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

Keywords: antibabesial activity, Arcangelisia flava, protoberberine alkaloid

JOURNAL FREE ACCESS

2005 Volume 67 Issue 2 Pages 223-227

DOI https://doi.org/10.1292/jvms.67.223

Details

Abstract

Bioassay-guided fractionation of the boiled extract from the stems of Arcangelisia flava led to the isolation of palmatine (1), berberine (2), jatrorrhizine (3), dihydroberberine (4) and 20-hydroxyecdysone (5). The chemical structures of these compounds were elucidated on the basis of their chemical and spectral evidence. The isolated compounds were evaluated for their growth inhibiting effects on Babesia gibsoni in culture for a week. Compounds (1-4) showed significant inhibitions at concentrations from 100 to 1.0 μg/ml, while compound 5 at a concentration of 100 μg/ml, only.

Corresponding author

Register with J-STAGE for free!