Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
FULL PAPER
Effects of Hyaluronic Acid on Ca2+ Influx, Lactate Dehydrogenase Activity, and Cyclic AMP Synthesis in Canine Ejaculated Sperm during In Vitro Capacitation
Eiichi KAWAKAMIYoshiro NISHIZONOChihiro MIYATATaichi HIRANOTatsuya HORIToshihiko TSUTSUI
Author information
JOURNALS FREE ACCESS

2006 Volume 68 Issue 2 Pages 119-123

Details
Abstract

The effects of hyaluronic acid, which comprises the cumulus intercellular matrix, on Ca2+ influx, lactate dehydrogenase (LDH) activity, and cyclic AMP synthesis in canine sperm during capacitation was investigated. Ejaculated sperm were collected from 10 Beagle dogs and the sperm were incubated for 4 hr in Eagle's MEM containing 10 μg/ml of hyaluronic acid. The percentages of actively motile sperm, hyperactivated sperm (HA-sperm), acrosome-reacted sperm (AR-sperm), and sperm labeled with fluoresceinated Ca2+ indicator (Ca2+-labeled sperm) were evaluated to assess Ca2+ influx into the sperm. LDH activity and cAMP concentration were measured in homogenized sperm. The mean percentages of motile sperm, HA-sperm, and Ca2+-labeled sperm in the MEM containing hyaluronic acid were higher than in the control medium (P<0.05, 0.05, and 0.01, respectively), but there was no difference between the percentages of AR-sperm. Mean LDH activity and mean cAMP concentration were also significantly higher than the control values (P<0.05). The percentages of HA-sperm correlated with those of Ca2+-labeled sperm (r2=0.810). The results indicate that hyaluronic acid increases Ca2+ influx, LDH activity, and cAMP synthesis in canine ejaculated sperm during capacitation in vitro.

Information related to the author

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
Previous article Next article
feedback
Top