Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Clinical Pathology
Crossreactivity of Antibodies to Canine CD25 and Foxp3 and Identification of Canine CD4+CD25 +Foxp3+ Cells in Canine Peripheral Blood
Takuya MIZUNORyoichi SUZUKISaori UMEKIMasaru OKUDA
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Keywords: canine, CD25, Foxp3, Treg
JOURNAL FREE ACCESS

2009 Volume 71 Issue 12 Pages 1561-1568

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Abstract

The importance of CD4+CD25+Foxp3 + regulatory T cells (Treg cells) in immune response is increasingly being recognized. However, to date, only a few studies on these cells have been conducted in canine species, partly because of the unavailability of appropriate antibodies to detect this cell population. In this study, the crossreactivities of anti-human CD25 antibody (clone ACT-1) and anti-mouse Foxp3 antibody (clone FJK16s) to canine CD25 and Foxp3, respectively, were confirmed using cell lines overexpressing either of these genes. By using these antibodies, we determined if CD4+, CD25+, or Foxp3+ cells were present in unstimulated canine peripheral blood mononuclear cells (PBMCs) and in concanavalin A (ConA)-stimulated PBMCs. CD25+ cells were not detected in unstimulated canine PBMCs, unlike in human or mouse PBMCs. ConA stimulation of canine PBMCs induced Foxp3 and CD25 expression in CD4+ and CD4- cells. These data indicate that the activation of canine lymphocytes might induce the production of adaptive Treg cells, as observed in humans.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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