Production of Ascaris suum As14 Protein and Its Fusion Protein with Cholera Toxin B Subunit in Rice Seeds

Tomoko NOZOYE; Fumio TAKAIWA; Naotoshi TSUJI; Takashi YAMAKAWA; Takeshi ARAKAWA; Yoshihiro HAYASHI; Yasunobu MATSUMOTO

doi:10.1292/jvms.71.995

Parasitology

Production of Ascaris suum As14 Protein and Its Fusion Protein with Cholera Toxin B Subunit in Rice Seeds

Tomoko NOZOYE, Fumio TAKAIWA, Naotoshi TSUJI, Takashi YAMAKAWA, Takeshi ARAKAWA, Yoshihiro HAYASHI, Yasunobu MATSUMOTO

Author information

Tomoko NOZOYE
Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Fumio TAKAIWA
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences
Naotoshi TSUJI
Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization
Takashi YAMAKAWA
Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Takeshi ARAKAWA
Division of Molecular Microbiology, Center of Molecular Biosciences, University of the Ryukyus
Yoshihiro HAYASHI
Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Yasunobu MATSUMOTO
Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Keywords: Ascaris suum 14-kDa surface antigen (As14), cholera toxin B subunit (CTB), edible vaccine, glutelin-B promoter, rice seed

JOURNAL FREE ACCESS

2009 Volume 71 Issue 7 Pages 995-1000

DOI https://doi.org/10.1292/jvms.71.995

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Abstract

Plants are attractive vaccine production and oral delivery systems. Cereals are excellent candidate for edible vaccines, which can express and store high levels of proteins for extended periods of time without degradation. In this study, we produced a 14-kDa protective surface antigen of Ascaris suum L3 larvae and its fusion chimera with a mucosal carrier molecule cholera toxin B subunit (CTB) in rice (Oryza sativa L.) under the control of the endosperm-specific glutelin-B promoter. We found that the recombinant protein expression levels reached 1.5 μg per seed, a comparably high amount as compared to previously reported transgenic rice expression experiments. Potentials of transgenic rice plants as a source of oral vaccines against swine roundworm are discussed.

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