Phenotyping of Sympathetic Chain Ganglia (SChG) Neurons in Porcine Colitis

Abstract

Colonic inflammation involves the changes in chemical coding of not only sensory but also enteric and sympathetic prevertebral neurons innervating inflamed target tissue. Since the sympathetic chain ganglia neurons (SChG) affect colonic function, the purpose of our study was to determine the precise location of the SChG neurons innervating the porcine descending colon, define their chemical coding and answer the question of whether paraformaldehyde-induced colitis in female pigs is able to change the chemical phenotype of the perikarya. Using the Fast Blue (FB) retrograde tracing, the greatest concentration of the (FB⁺) perikarya was revealed in L₃ ganglia. Applying double immunohistochemistry, we detected a strongly catecholaminergic character and in majority neuropeptide Y (NPY)-positive staining of the colon-projecting FB⁺ neurons both in the control and the inflamed animals. Furthermore, colonic inflammation caused the significant increase in density of galanin- (P<0.001), somatostatin- (P<0.005), leu5-enkephalin- (P<0.05), substance P- (P<0.005), and calcitonin gene-related peptide- (P<0.005) immunoreactive (-IR) nerve fibers surrounding the FB⁺ perikarya, while the density of nitric oxide synthase-IR and NPY-IR fibers remained unaffected compared to the control animals. The increase in visible immunofluorescent detection of neuropeptide-containing fibers could possibly be caused by enhanced demand and release of neuropeptides that modulate intestinal immune response and regulate the gastrointestinal activity through inflammatory mediators.