Prevalence of Murine Norovirus Infection in Korean Laboratory Animal Facilities

Jong Rhan KIM; Seung Hyeok SEOK; Dong Jae KIM; Min-Won BAEK; Yi-Rang NA; Ju-Hee HAN; Tae-Hyun KIM; Jae-Hak PARK; Patricia V. TURNER; Doo Hyun CHUNG; Byeong-Cheol KANG

doi:10.1292/jvms.10-0226

Laboratory Animal Science

Prevalence of Murine Norovirus Infection in Korean Laboratory Animal Facilities

Jong Rhan KIM ^†, Seung Hyeok SEOK ^†, Dong Jae KIM ^†, Min-Won BAEK ^†, Yi-Rang NA ^†, Ju-Hee HAN ^†, Tae-Hyun KIM ^†, Jae-Hak PARK ^†, Patricia V. TURNER ^†, Doo Hyun CHUNG ^†, Byeong-Cheol KANG ^†

Author information

Jong Rhan KIM ^†
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University
Biomedical Research Institute, Seoul National University Hospital
Seung Hyeok SEOK ^†
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University
Dong Jae KIM ^†
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University
Min-Won BAEK ^†
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University
Yi-Rang NA ^†
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University
Ju-Hee HAN ^†
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University
Tae-Hyun KIM ^†
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University
Jae-Hak PARK ^†
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University
Patricia V. TURNER ^†
Department of Pathology, Ontario Veterinary College, University of Guelph
Doo Hyun CHUNG ^†
Biomedical Research Institute, Seoul National University Hospital
Byeong-Cheol KANG ^†
Biomedical Research Institute, Seoul National University Hospital
Graduate School of Immunology, College of Medicine, Seoul National University
Author contributions

†These authors contributed equally: Jong Rhan KIM, Seung Hyeok SEOK, Dong Jae KIM, Min-Won BAEK, Yi-Rang NA, Ju-Hee HAN, Tae-Hyun KIM, Jae-Hak PARK, Patricia V. TURNER, Doo Hyun CHUNG, Byeong-Cheol KANG

Keywords: genetically modified mouse, Korea, murine norovirus, prevalence

JOURNAL FREE ACCESS

2011 Volume 73 Issue 5 Pages 687-691

DOI https://doi.org/10.1292/jvms.10-0226

View "Advance Publication" version (December 22, 2010).

Details

Abstract

Currently, murine noroviruses (MNV) are the most prevalent viral pathogens identified in laboratory animal facilities. While several reports exist concerning the prevalence of MNV in North American research facilities, very few reports are available for other parts of the world, including Korea. This study evaluated the prevalence of MNV infection in 745 murine sera collected from 15 animal facilities in Korea by enzyme linked immunosorbent assay (ELISA). Positive cases were subcategorized by murine strain/genetics, housing environments and animal sources. In summary, 6.6% of inbred/outbred mice purchased from commercial vendors were seropositive, 9.6% of in-house colonies were seropositive and 27.0% of genetically modified mice (GMM) were seropositive. Partial gene amplification of fecal isolates from infected animals showed that they were homologous (100%) with MNV-4.

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