Molecular Epidemiological Survey of the Babesia gibsoni cytochrome b Gene in Western Japan

Masato SAKUMA; Kenta FUKUDA; Katsuyoshi TAKAYAMA; Yukuharu KOBAYASHI; Takako Shimokawa MIYAMA; Asuka SETOGUCHI; Yasuyuki ENDO

doi:10.1292/jvms.12-0140

Internal Medicine

Molecular Epidemiological Survey of the Babesia gibsoni cytochrome b Gene in Western Japan

Masato SAKUMA, Kenta FUKUDA, Katsuyoshi TAKAYAMA, Yukuharu KOBAYASHI, Takako Shimokawa MIYAMA, Asuka SETOGUCHI, Yasuyuki ENDO

Author information

Masato SAKUMA
Laboratory of Small Animal Internal Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Korimoto, Kagoshima, Kagoshima 890–0065, Japan
Kenta FUKUDA
ADTEC Co., Ltd., 1693–6 Yokkaichi, Usa, Oita 879–0471, Japan
Katsuyoshi TAKAYAMA
ADTEC Co., Ltd., 1693–6 Yokkaichi, Usa, Oita 879–0471, Japan
Yukuharu KOBAYASHI
ADTEC Co., Ltd., 1693–6 Yokkaichi, Usa, Oita 879–0471, Japan
Takako Shimokawa MIYAMA
Veterinary Teaching Hospital, Kagoshima University, 1–21–24 Korimoto, Kagoshima, Kagoshima 890–0065, Japan
Asuka SETOGUCHI
Laboratory of Small Animal Internal Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Korimoto, Kagoshima, Kagoshima 890–0065, Japan
Yasuyuki ENDO
Laboratory of Small Animal Internal Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Korimoto, Kagoshima, Kagoshima 890–0065, Japan

Keywords: Babesia gibsoni, cytochrome b gene, drug-resistance, epidemiological survey

JOURNAL FREE ACCESS

2012 Volume 74 Issue 10 Pages 1341-1344

DOI https://doi.org/10.1292/jvms.12-0140

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Abstract

In this study, we conducted a survey of the cytochrome b (cytb) gene of Babesia gibsoni (B. gibsoni) isolated from clinical cases to determine the prevalence of potential atovaquone (ATV)-resistant variants. Ninety-two blood samples were collected from naturally B. gibsoni infected dogs. The cytb nucleotide sequence was determined by direct sequencing. Twelve non-synonymous amino acid substitutions were identified in cytb. The principal ATV-resistant substitution, M121I, was detected in three cases. This survey determined that potentially ATV-resistant B. gibsoni strains are present in dogs in Japan.

Corresponding author

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