Molecular-Based Identification of Yeasts Isolated from Bovine Clinical Mastitis in Japan

Tomohito HAYASHI; Takashi SUGITA; Eiji HATA; Ken KATSUDA; Enshi ZHANG; Yoshio KIKU; Kazue SUGAWARA; Tomomi OZAWA; Tomoko MATSUBARA; Takaaki ANDO; Tetsu OBAYASHI; Takaaki ITO; Takahiro YABUSAKI; Katsunori KUDO; Hiroshi YAMAMOTO; Masateru KOIWA; Toshio OSHIDA; Yuichi TAGAWA; Kazuhiro KAWAI

doi:10.1292/jvms.12-0362

Bacteriology

Molecular-Based Identification of Yeasts Isolated from Bovine Clinical Mastitis in Japan

Tomohito HAYASHI, Takashi SUGITA, Eiji HATA, Ken KATSUDA, Enshi ZHANG, Yoshio KIKU, Kazue SUGAWARA, Tomomi OZAWA, Tomoko MATSUBARA, Takaaki ANDO, Tetsu OBAYASHI, Takaaki ITO, Takahiro YABUSAKI, Katsunori KUDO, Hiroshi YAMAMOTO, Masateru KOIWA, Toshio OSHIDA, Yuichi TAGAWA, Kazuhiro KAWAI

Author information

Tomohito HAYASHI
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
Takashi SUGITA
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo 204–8588, Japan
Eiji HATA
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
Ken KATSUDA
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
Enshi ZHANG
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo 204–8588, Japan
Yoshio KIKU
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
Kazue SUGAWARA
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
Tomomi OZAWA
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
Tomoko MATSUBARA
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
Takaaki ANDO
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069–8501, Japan
Tetsu OBAYASHI
Tokachi NOSAI, Taiki, Hokkaido 089–2106, Japan
Takaaki ITO
Aichi NOSAI, Okazaki, Aichi 444–0816, Japan
Takahiro YABUSAKI
Ishikari NOSAI, Ebetsu, Hokkaido 069–0055, Japan
Katsunori KUDO
Ishikari NOSAI, Ebetsu, Hokkaido 069–0055, Japan
Hiroshi YAMAMOTO
Okhotsk NOSAI, Kitami, Hokkaido 099–0879, Japan
Masateru KOIWA
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069–8501, Japan
Toshio OSHIDA
School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252–5201, Japan
Yuichi TAGAWA
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
Kazuhiro KAWAI
School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252–5201, Japan

Keywords: clinical mastitis, Japan, molecular analysis, yeast

JOURNAL FREE ACCESS

2013 Volume 75 Issue 3 Pages 387-390

DOI https://doi.org/10.1292/jvms.12-0362

Browse “Advance online publication” version

Details

Abstract

This study analyzed molecular-based identification of yeasts that associated with bovine clinical mastitis in Japan. Over 3,200 quarter milk samples from Holstein dairy cows collected in 2011 on Hokkaido and Honshu islands were examined. Yeast isolates were characterized by polymerase chain reaction amplification and sequencing of the D1/D2 region of the 26S rDNA. Molecular characterization confirmed that Candida spp. and Pichia spp. were most frequently isolated species. Our molecular analysis of mastitic milk samples demonstrated the prevalence of Pichia kudriavzevii(22/58) and Candida tropicalis(14/58). In addition, we demonstrated that molecular analysis of the D1/D2 region of the 26S rDNA is a rapid and reliable method for identifying clinically significant yeasts in dairy hygiene, including potentially new or emerging pathogenic species.

Corresponding author

Register with J-STAGE for free!