Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Pharmacology
Negative Inotropic Effect of Carbachol and Interaction between Acetylcholine Receptor-Operated Potassium Channel (K.ACh Channel) and GTP Binding Protein in Mouse Isolated Atrium ― A Novel Methodological Trial
Muneyoshi OKADAChihiro NOMAHideyuki YAMAWAKIYukio HARA
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2013 Volume 75 Issue 3 Pages 377-380

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Abstract

Interaction between acetylcholine receptor-operated potassium channel (K.ACh channel) and GTP binding protein was examined by an immunoprecipitation-Western blotting system in mouse isolated atrium. The carbachol-induced negative inotropic action in indomethacin-pretreated mouse atrium was significantly inhibited by a K.ACh channel blocker, tertiapin or atropine. Kir3.1 K.ACh channel (Kir3.1) was immunoprecipitated with a mouse anti-Kir3.1 antibody. Coprecipitating Gβ with Kir3.1, detected by Western blotting, was significantly augmented by carbachol. Atropine, but not tertiapin, significantly inhibited the carbachol-induced coprecipitating Gβ with Kir3.1. The data indicate that immunoprecipitation with Kir3.1 and Western blotting of Gβ system is a useful method for assessing interaction between K.ACh channel and GTP binding protein in mouse atrium.

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https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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