Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin

Seishiro CHIKAZAWA; Yasutomo HORI; Fumio HOSHI; Kazutaka KANAI; Naoyuki ITO; Jun SATO; Koichi ORINO; Kiyotaka WATANABE; Sei-ichi HIGUCHI

doi:10.1292/jvms.12-0324

Internal Medicine

Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin

Seishiro CHIKAZAWA, Yasutomo HORI, Fumio HOSHI, Kazutaka KANAI, Naoyuki ITO, Jun SATO, Koichi ORINO, Kiyotaka WATANABE, Sei-ichi HIGUCHI

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Keywords: canine, ferritin, sandwich ELISA

JOURNAL FREE ACCESS

2013 Volume 75 Issue 4 Pages 515-517

DOI https://doi.org/10.1292/jvms.12-0324

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Abstract

We established a homologous sandwich enzyme-linked immunosorbent assay (ELISA) to measure serum levels of canine ferritin. Our assay uses a rabbit anti-canine heart ferritin polyclonal antibody, and canine heart ferritin as a standard. Serum ferritin concentration in healthy dogs (n=163) was 789 ± 284 ng/ml (mean ± standard deviation), a value higher than reported previously. Confidence levels relating to repeatability, dilution and recovery for this method were high. Therefore, we believe our developed sandwich ELISA will be effective in evaluating serum levels of canine ferritin.

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