Culture on a Fragmin/protamine-Coated Plate Suppresses the Collagen Type IαI and TGF-β1 mRNA Expression of Rat Hepatic Stellate RI-T Cells

Hitomi SEKIGUCHI; Natsuko HEMMI; Takehiro MAKI; Aisa OZAWA; Erina KADOWAKI; Junichi KAMIIE; Masako YAMAMOTO; Kazuyoshi ARISHIMA; Motoharu SAKAUE

doi:10.1292/jvms.12-0396

Biochemistry

Culture on a Fragmin/protamine-Coated Plate Suppresses the Collagen Type IαI and TGF-β1 mRNA Expression of Rat Hepatic Stellate RI-T Cells

Hitomi SEKIGUCHI, Natsuko HEMMI, Takehiro MAKI, Aisa OZAWA, Erina KADOWAKI, Junichi KAMIIE, Masako YAMAMOTO, Kazuyoshi ARISHIMA, Motoharu SAKAUE

Author information

Hitomi SEKIGUCHI
Department of Anatomy II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan
Natsuko HEMMI
Department of Anatomy II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan
Takehiro MAKI
Department of Anatomy II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan
Aisa OZAWA
Department of Anatomy II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan
Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Erina KADOWAKI
Department of Anatomy II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan
Junichi KAMIIE
Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan
Masako YAMAMOTO
Department of Anatomy II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan
Kazuyoshi ARISHIMA
Department of Anatomy II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan
Motoharu SAKAUE
Department of Anatomy II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoh-ku, Sagamihara 252–5201, Japan

Keywords: collagen type IαI, Fragmin/protamine microparticles, hepatic stellate cell, TGF- β1

JOURNAL FREE ACCESS

2013 Volume 75 Issue 5 Pages 553-559

DOI https://doi.org/10.1292/jvms.12-0396

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Abstract

Hepatic stellate cells (HSCs) intracellularly preserve vitamin A in the normal liver. When the liver is damaged, HSCs transform into myofibroblast-like cells, and then proliferate and increase their expression of collagen. Cultured on a plastic plate, HSCs spontaneously activate. To maintain HSCs in a quiescent state with low expression of collagen, coating methods with extracellular matrixes (ECMs) such as Matrigel-coating or laminin-rich coating are commonly used for HSC cultivation. Kishimoto et al. [14] reported that Fragmin®/protamine microparticles (F/P-MPs) have the ability to absorb heparin-binding cytokines like ECMs. Therefore, we examined whether the cultivation on an F/P-MPs-coated plate maintains the quiescent state of RI-T cells (derived from rat HSCs) including the suppression of collagen expression. We found that the mRNA levels of collagen type IαI and TGF-β1 in RI-T cells were significantly suppressed in the cultivation on F/P-MPs-coated plates compared to cultures on noncoated and Matrigel-coated plates. We conclude that the F/P-MPs coating method is useful for maintaining with low expressions of collagen IαI and TGF-β 1 mRNA levels in HSCs.

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